Phytochemical Constituents, Antioxidant Activities and GCMS Analysis of the Methanol Fruit Extract of Thespian Garckeana (Malvaceae)
Keywords:
Phytochemical, Thespian garckeana, Antioxidant models, GC-MS AnalysisAbstract
Africa is home to the tropical fruit plant known as Thespian garckeana. Its seeds are thrown, but its edible fruit is used as food or as herbal remedy. One kilogram of the powdered material was utilized, and a Soxhlet extraction method was performed to obtain the plant material's methanol crude extract. Alkaloid was not detected in the methanol extract of T. garckeana, despite screening for the presence of secondary metabolites in varied amounts. Folin-Ciocalteu, Aluminum Chloride colorimetric tests, and Folin's Phenol reagent were used to determine the total phenolics, total flavonoids, and total tannin content (TPC, TFC, and TTC). For total phenolics, total flavonoids, and total tannins, the internal standards were gallic acid, quercetin, and tannic acid. TPC was found at the highest concentration in the methanol extract of T. garckeana (54.00 ± 0.02 (mgGAE/g)), followed by TTC (24.67 ± 0.01 (mgTAE/g)) and TFC (11.99 ± 0.01 (mgQE/g)) in the quantitative study.The fruit (T. garckeana) demonstrated good antioxidant activity because the percentage inhibition increased as the concentration increased. The results of the three antioxidant models were reported as follows: (78.50 ± 0.01) for DPPH scavenging activity, (100.56 ± 0.03) for total antioxidant capacity, and (82.68 ± 0.01) for nitric oxide. The Gas Chromatography-Mass Spectrometry (GC-MS) was done and fifteen peaks were detected from the GC-MS spectra, corresponding to fifteen bioactive chemicals and the majority of the chemicals that were found were esters and carboxylic acids. According to this study, T. garckeana fruit possesses a substantial abundance of phenolic compounds and strong antioxidant properties in vitro. The fruit may potentially be used as a source of traditional medicine because the bioactive chemicals have key biological properties.Downloads
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[1] Celiktar, O. Y., Girgin, G., Orhan, H., Nichers,H. J., Bedir, E. and Sukan, F. V. (2007). Screening of free radical scavenging capacity and antioxidant activities of Rosmarinus officinalis extracts with focus on location and harvesting times. European Food Research and Technology, 24: 443-451.
[2] Gamiotea-Tirro, D., Cuesta-Rubio, O., Prieto-Gonzalez, S., DeSimone, F., Passi, S. and Rastrelli, L. (2004). Antioxidative constituents from the leaves of Hypericum syphelioides. Journal of Natural Products, 67: 869-871.
[3] Moosmann, B., and Behl, C. (1999). The antioxidant neuroprotective effects of estrogens and phenolic compounds are independent from their estrogenic properties. Proceedings of the National Academy of Sciences, 96(16), 8867-8872.
[4] Gomes, C.A., Girao da Cruz, T., Andrade, J.L., Milhazes, N., Borges, F., and Marques, M.P.M. (2003). Anti-cancer activity of phenolic acids of natural or synthetic origin: a structure activity study. Journal of Medicinal Chemistry, 46(25): 5395-5401.
[5] Salisbury, B. H. and Tadi, P. (2021). 5- alpha reductase inhibitors. In Stat pearls internet. Stat Pearls Publishing, Treasure Island (FL), 4-5
[6] Anderson, R. F., Amarasinghe, C., Fisher, L, J., Mak, W. B., and Packer, J. E. (2000). Reduction in free-radical-induced DNA strand breaks and base damage through fast chemical repair by flavonoids. Free Radicals Research, 33 (1):91-103.
[7] Cragg, G.M. and Newman, D.J. (2005). Biodiversity: A continuing source of novel drug leads. Pure and Applied Chemistry 77 (1):7-24.
[8] Aniya, Y. (2002). Antioxidants in traditional foods and medicinal plants from Okinawa. In D. Itokazu, H. Sho, & Y. Nakahara. Proceeding of Okinawa International Conference on Longevity. P.50.Naha: OICL.
[9] Ugorji, C.O., Ihedioha, J.N., Agbo, M.O., Ekere N.R., Nwafor, F.I., Odoemelam, E.I. (2019). Antimicrobial and Antioxidant Studies of the Leaf Extract and Fractions of Sabicea brevipes Wernham (Rubiaceae), International Journal of Biochemistry Research & Review, 27 (2): 9-10
[10] Fukumoto, L., Mazza, G. (2000). Assessing antioxidant and prooxidant activities of phenolic compounds. J. Agric. Food Chem., 48: 3597-3604
[11] Harbourne, J. B. (1984). Textbook of Phytochemical Methods and Guide to Modern Techniques of Plant Analysis 2nd Edition. Chapman and Hall Ltd. London, 279.
[12] Hassan, V., Ali, S., Fatemeh, M.K., Hossein, D., Mir, B.B. (2015). Cytotoxicity, antioxidant activity of phenolic content of eight fern species from north of Iran. Pharmaceutical Sciences, 21: 18-24.
[13] Brand-Williams, W., Cuvelier, M. E., and Berset, C. (1995). “Use of a free radical method to evaluate antioxidant activity,” Food Science and Technology, 28 (1): 25–30.
[14] Lallianrawna, S., Muthukumaran, R., Vanlalhruaii, R., Gurusubramanian, G. and Kumar, N.S. (2013). Determination of total phenolic content, total flavonoid content and total antioxidant capacity of Ageratina adenophora (Spreng.) King & H. Rob. Science Vissions, 13(4): 149-156.
[15] Hymer, C.B., Cheever, K.L., Butler, M.A., Brown K.K. (2003). Procedure for the quantification of the biomarker (2-methoxyethoxy) acetic acid in human urine samples, Journal of Chromatography B, 795 (1): 1570-1573.
[16] Kawuri, R., Darmayasa, I. B. G. (2019). Bioactive compound from extract filtrat streptomyces as biocontrol of vibriosis on larvae of Macrobrachium rosenbergii shrimps. Journal of Biosciences, 26(1), 15-25.
[17] Bashir, L., Saidu, S., Amos, S., Onikanni D., Yunusa, O. I., Abdulhakeem, R. A., Halimat, Y. L., Femi, O., Ali, A. J., Gaber, E. B., Shukurat, B. B. , Gomaa, M., Clara, M. G. L., Alexander T.H., Wu, H. H., Carlos, A. C. (2022). Preclinical anti-inflammatory and antioxidant effects of Azanza garckeana in STZ-induced glycemic-impaired rats, and pharmacoinformatics of it major phytoconstituents. Biomedicine & Pharmacotherapy, 15 (2): 113-196.
[18] Majinda, R. and Abubakar, M. (2016). Phytochemical constituents and antimicrobial activity of Albizia adianthifolia. International Journal of Pharmacognosy Phytochemistry Research, 3(3): 1-7.
[19] Asghar, S. F. and Choudahry, M. I. (2011). Gas chromatography-mass spectrometry (GC-MS) analysis of petroleum ether extract (oil) and bioassays of crude extract of Iris germania. International Journal Genetic Molecular Biology, 3(7): 95 - 100.
[20] Enas, J. K. and Duha, A. A. (2014). Phytochemical Characterization using GC-MS Analysis of Methanolic Extract of Moringa oleifera (Family Moringaceae) plant cultivated in Iraq. Journal of Chemistry of Materials, 6(5): 9-26.
[21] Momodu, I., Okungbowa, E., Agoreyo, B., Maliki, M. (2022). Gas Chromatography Mass Spectrometry Identification of Bioactive Compounds in Methanol and Aqueous Seed Extracts of Azanza garckeana Fruits. Nigerian Journal of Biotechnology, 38, 25-38.
[22] Teres, S., Barcelo-Coblijn, G., Benet, M., Alvarez, R., Bressani, R., Halver, J.E. (2008). Oleic acid content is responsible for the reduction in blood pressure induced by olive oil. Pro. National Academy of Science USA; 105:13811-13816.
[23] Baba, H., Ibe, A.C. and Kashimawo, A.J. (2021). Phytochemical Evaluation and GC-MS Profiling of the Dichloromethane and Ethanol extracts of Ocimum gratissimum L. and Lasianthera africana. BEAUV. JOPAT 20 (2) 640- 655.
[24] Hakkim F.L., Shankar C.G., Girija S. (2007). Chemical composition and antioxidant property of holy basil (Ocimum sanctum, L.) leaves, stems, and inflorescence and their in vitro callus cultures. Journal of Agriculture Food Chemistry 55: 9109-9117.
[25] Maroyi, A. (2017). Azanza garckeana Fruit Tree: Phytochemistry, Pharmacology, Nutritional and Primary Healthcare Applications as Herbal Medicine: A Review. Research Journal Medicinal Plants, 11 (4): 115-123.
[26] Ahmed, R.H., El Hassan, M.S., El Hadi, H.M. (2016). Potential capability of Azanza garckeana fruits aqueous extract on enhancement of iron absorption in Wistar albino rats. International Journal of Advanced Research Biological Sciences, 3, 245-250.
[27] Alfred M. (2017). Azanza garckeana fruit tree: Phytochemistry, pharmacology, nutritional and primary healthcare applications as herbal medicine: a review. Research Journal of Medicinal Plants, 11, 115-123.
[28] Trease, E., and Evans, W. C. (2002). Phenols and phenolic glycosides. In: Textbook of Pharmacology 15th ed. London: Elsevier Science Ltd. 214-252.
[29] Meena A.S., Taksim, A., Mohammad, N.U., Senjuti, M.M., Sohel, R. (2013). Phytochemical screening and In-vitro determination of Antioxidant potential of methanolic extract of Streospermum chelonoids. Journal of Applied Pharmaceutical Sciences, 3 (3): 117-121.
[30] Rivière, C., Hong, V. N, Pieters, L., Dejaegher, B., Heyden, Y. V, Van, M. C. (2009). Polyphenols isolated from antiradical extracts of Mallotus metcalfianus. Phytochemistry; 70:86-94.
[31] Kumasaramy, Y., Byres, M., Cox, P.J., Jaspars M., Nahar, L., Sarker, S.D. (2007). Screening seeds of some Scottish plants for free radical scavenging activity. Phytotherapy Research, 21: 615-620.
[32] Wagg, S.S., Vadnere, G.P., Patil, A.V., Jain, S.K. (2012). In vitro free radical scavenging and antioxidant activity of Cicer arietinum L. (Fabaceae). International Journal of Pharmaceutical Technology Research CODEN (USA), 4(1): 343-350.
[33] Jennings, W. and Shibamoto, T. (1980). Qualitative Analysis of flavor and fragrance volatiles by Glass Capillary Gas Chromatography. 465 Seiten Academic Press, New York.
[34] Yu, F.R., Lian, X.Z. Guo, H.Y. McGuire, P.M. Li, R.D. Wang, R. Yu, F.H. (2005). Isolation and characterization of methyl esters and derivatives from Euphorbia kansui (Euphorbiaceae) and their inhibitory effects on the human SGC-7901 cells. Journal of Pharmaceutical Sciences, 8, 528- 535.
[35] Prabhadevi, V., Sahaya, S.S., Johnson M., Venkatramani, B., Janakiraman (2012). Phytochemical studies on Allamanda cathartica L. using GC-MS, Asian Pacific Journal of Tropical Biomedicine, 2 (2): 550-554
[36] Liang, T. and Liao, S. (1992). Inhibition of steroid 5 alpha-reductase by specific aliphatic unsaturated fatty acids. Biochemistry Journal, Pp. 557-562.
[37] Jean-Pierre, R., Henri, C., Pierre-Marie, M. (2002). Inhibition of type 1 and type 2 5 αreductase activity by free fatty acids, active ingredients of Permixon. Journal of Steroid Biochemistry Molecular Biology 82 (3): 233-239.
[38] Tomlinson, J. W. and Finney, J. (2008). Impaired glucose tolerance and insulin resistance are associated with increased adipose 11beta-hydroxysteroid dehydrogenase type 1 expression and elevated hepatic 5alphareductase activity. Diabetes and Metabolism Journal, 57(10): 2652-2660.
[39] Vassiliadi, D. A. and Barber, T. M. (2009). Increased 5 alpha-reductase activity and adrenocortical drive in women with polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism, 94(9): 3558-3566.
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